Hydrogen-ion-titration studies of pancreatic phospholipase A and its zymogen.

Hydrogen-Ion-Titration Studies of Pancreatic Phospholipase A and lts Zymogen

Hydrogen-Ion-Titration Studies of Pancreatic Phospholipase A and lts Zymogen

Hydrogen-Ion-Titration Studies of Pancreatic Phospholipase A and lts Zymogen

Identification of membrane dipeptidase as a major glycosyl-phosphatidylinositol-anchored protein of the pancreatic zymogen granule membrane, and evidence for its release by phospholipase A.

Membrane dipeptidase (EC 3.4.13.19) enzyme activity that is inhibited by cilastatin has been detected in pancreatic zymogen granule membranes of human, porcine and rat origin. Immunoelectrophoretic blot analysis of human and porcine pancreatic zymogen granule membranes with polyclonal antisera raised against the corresponding kidney membrane dipeptidase revealed that the enzyme is a disulphide-...

Rat GP-3 is a pancreatic lipase with kinetic properties that differ from cohpase-dependent pancreatic lipase

The pancreas contains three homologous proteins, colipase-dependent pancreatic lipase (PL) and two recently described pancreatic lipase-related proteins, PLRPl and PLRP2. Rat (r) PLRP2 was first identified as a zymogen granule membrane protein, GP-3. Subsequently, we showed that rPLRP2 could cleave fatty acids from triglycerides, but the kinetic properties of rPLRP2 have not been further invest...